Effect of Net Charge on DNA-Binding, Protein-Binding and Anticancer Properties of Copper(I) Phosphine-Diimine Complexes

Abstract The syntheses of [Cu(PPh 3 ) 2 (L)]NO and (L-SO Na)]NO were achieved through the reaction Cu(PPh NO equimolar amount ligands (L = 5,6-diphenyl-3-[2-pyridyl]-1,2,4-triazine; LSO Na 5,6-diphenyl-3-[2-pyridyl]-1,2,4-triazine-4,4?-disulfonic acid disodium salt). complexes characterized by NMR IR spectroscopy mass spectrometry. compounds exhibit similar absorption emission spectra, suggesting a electronic structure. Ct-DNA binding studies show strong influence net charge as Cu-L (positively charged) is able to bind ct-DNA while Cu-LSO (negatively not. affects thermodynamic kinetic parameters toward human serum albumin. HSA-binding was further investigated molecular docking, revealing different sites on HSA protein function charge. anticancer activities towards ovcar-3 hope-62 cancer cell lines are suggestive role for overall complexes. Interaction with DNA not major mechanism this class pharmacophore (anticancer agent) should be key consideration in design metal